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An oil immersion objective is an objective lens specially designed to be used in this way. ... Objectives with high power magnification have short focal lengths ...
With an optical microscope having a high numerical aperture and using oil immersion, the best possible resolution is 200 nm corresponding to a magnification of around 1200×. Without oil immersion, the maximum usable magnification is around 800×. For details, see limitations of optical microscopes.
Some microscopes make use of oil-immersion objectives or water-immersion objectives for greater resolution at high magnification. These are used with index-matching material such as immersion oil or water and a matched cover slip between the objective lens and the sample. The refractive index of the index-matching material is higher than air ...
Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
Oil immersion is a technique that can increase image resolution by immersing the lens and the specimen in oil with a high refractive index. Since light bends when it passes between media with different refractive indexes, by placing oil with the same refractive index as glass between the lens and the slide, two transitions between refractive ...
As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...
The practical limit to magnification with a light microscope is around 1300×. ... Use of an oil-immersion objective lens and a special immersion oil placed on a ...
In a high-resolution oil immersion lens, the maximum NA is typically 1.45, when using immersion oil with a refractive index of 1.52. Due to these limitations, the resolution limit of a light microscope using visible light is about 200 nm .
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