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A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...
For example, this could be transferring heat from a hot flow of liquid to a cold one, or transferring the concentration of a dissolved solute from a high concentration flow of liquid to a low concentration flow. The counter-current exchange system can maintain a nearly constant gradient between the two flows over their entire length of contact ...
Countercurrent distribution, therefore, is a method of using a series of vessels (separatory funnels) to separate compounds by a sequence of liquid-liquid extraction operations. Contrary to liquid-liquid extraction, in the CCD instruments the upper phase is decanted from the lower phase once the phases have settled.
Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly employed for the purification of antibodies in the biopharmaceutical industry [ 1 ] as well as in research and development.
The ability of the medium to swell in liquid is based on the cross-linking of these substances, the pH and the ion concentrations of the buffers used. [ 26 ] Incorporation of high temperature and pressure allows a significant increase in the efficiency of ion chromatography, along with a decrease in time.
Salt metathesis is a common technique for exchanging counterions. The choice of reactants is guided by a solubility chart or lattice energy. HSAB theory can also be used to predict the products of a metathesis reaction. Salt metathesis is often employed to obtain salts that are soluble in organic solvents.
The largest ocean current is the Antarctic Circumpolar Current (ACC), a wind-driven current which flows clockwise uninterrupted around Antarctica. The ACC connects all the ocean basins together, and also provides a link between the atmosphere and the deep ocean due to the way water upwells and downwells on either side of it.
The process was termed "counter-current liquid-liquid extraction." [5] Martin and Synge described the theory of this technique in reference to continuous fractional distillation described by Randall and Longtin. [6]