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Different colors of bromothymol blue at marked pH conditions. Bromothymol blue may be used for observing photosynthetic activities, or as a respiratory indicator (turns yellow as CO 2 is added). [7] [8] A common demonstration of BTB's pH indicator properties involves exhaling through a tube into a neutral solution of BTB.
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The observed spectrum (green) is the sum of the spectra of HA (gold) and of A − (blue), weighted for the concentration of the two species. When a single indicator is used, this method is limited to measurements in the pH range p K a ± 1, but this range can be extended by using mixtures of two or more indicators.
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Thymol blue (thymolsulfonephthalein) is a brownish-green or reddish-brown crystalline powder that is used as a pH indicator.
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