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Phenol is a component in liquid–liquid phenol–chloroform extraction technique used in molecular biology for obtaining nucleic acids from tissues or cell culture samples. Depending on the pH of the solution either DNA or RNA can be extracted.
The name "cresol" is an adduct of phenol and their ... Lysol" has been used as a generic trademark to refer to such a cresol soap solution and remains used as such in ...
In 1834, German chemist Friedlieb Ferdinand Runge discovered a phenol, also known as carbolic acid, which he derived in an impure form from coal tar.In August 1865, Joseph Lister applied a piece of lint dipped in carbolic acid solution to the wound of an eleven-year-old boy at Glasgow Royal Infirmary, who had sustained a compound fracture after a cart wheel had passed over his leg.
The ideal concentration for the manufacture of disinfectants is a phenol content of 15% on the product. In this way, when the disinfectant is prepared for use, it will always have a final concentration of phenols higher than 1%, which is used for cleaning and disinfection. [citation needed]
Phenol extraction is a laboratory technique that purifies nucleic acid samples using a phenol solution. Phenol is common reagent in extraction because its properties allow for effective nucleic acid extraction, particularly as it strongly denatures proteins, it is a nucleic acid preservative, and it is immiscible in water.
TCP is used as the subject of a song by the same name on The Boys' 1978 album Alternative Chartbusters written by Honest John Plain. TCP is mentioned in the song " Obsessions " by Suede . In the Black Mirror episode Metalhead (series 4 episode 5), a broken bottle of TCP is seen being pulled out of a backpack in advance of minor surgery.
Phenol red exists as a red crystal that is stable in air. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. [1] It is a weak acid with pK a = 8.00 at 20 °C (68 °F). A solution of phenol red is used as a pH indicator, often in cell culture.
TRIzol reagent contains guanidinium thiocyanate and phenol.. TRIzol is a widely used [1] chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.
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