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Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
The Gram stain is used by microbiologists to place bacteria into two main categories, Gram-positive (+) and Gram-negative (-). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and Gram-negative bacteria have a thin layer of peptidoglycan.
Haemophilus influenzae (formerly called Pfeiffer's bacillus or Bacillus influenzae) is a Gram-negative, non-motile, coccobacillary, facultatively anaerobic, capnophilic pathogenic bacterium of the family Pasteurellaceae. The bacteria are mesophilic and grow best at temperatures between 35 and 37 °C. [1]
In Berlin, in 1884, Gram developed a method for distinguishing between two major classes of bacteria. [1] This technique, known as Gram staining, continues to be a standard procedure of medical microbiology. This work gained Gram an international reputation. The staining method later played a major role in classifying bacteria. Gram was a ...
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
Staphylococci spp. are a genus of gram positive cocci of 0.5 - 1 μm diameter. [citation needed] Staphylococcus pseudintermedius is a non-motile and non-spore forming, facultatively anaerobic bacterium. [citation needed] It appears primarily as grape-like clusters morphologically, but can also be seen as individual or paired cocci.
It is Gram-positive by Gram staining, but Mycobacterium leprae was traditionally stained with carbol fuchsin in the Ziehl–Neelsen stain. Because the bacilli are less acid-fast than Mycobacterium tuberculosis (MTB), the Fite-Faraco staining method, which has a lower acid concentration, is used now. [9] [10] In size and shape, it closely ...
Veillonella are Gram-negative bacteria (Gram stain pink) anaerobic cocci, unlike most Bacillota, which are Gram-positive bacteria. [1] This bacterium is well known for its lactate fermenting abilities. It is a normal bacterium in the intestines and oral mucosa of mammals.