enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. Elution - Wikipedia

    en.wikipedia.org/wiki/Elution

    Elution then is the process of removing analytes from the adsorbent by running a solvent, called an "eluent", past the adsorbent–analyte complex. As the solvent molecules "elute", or travel down through the chromatography column, they can either pass by the adsorbent–analyte complex or displace the analyte by binding to the adsorbent in its ...

  3. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    The organic solvent is called also a modifier, since it is added to the aqueous solution in the mobile phase in order to modify the polarity of the mobile phase. Water is the most polar solvent in the reversed phase mobile phase; therefore, lowering the polarity of the mobile phase by adding modifiers enhances its elution strength.

  4. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    In reversed-phase chromatography, solvent A is often water or an aqueous buffer, while B is an organic solvent miscible with water, such as acetonitrile, methanol, THF, or isopropanol. In isocratic elution, peak width increases with retention time linearly according to the equation for N, the number of theoretical plates.

  5. Aqueous normal-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Aqueous_normal-phase...

    Retention decreases as the amount of polar solvent in the mobile phase increases. In reversed phase chromatography, the most polar compounds elute first with the more nonpolar compounds eluting later. The mobile phase is generally a mixture of water and miscible polarity-modifying organic solvent, such as methanol, acetonitrile or THF ...

  6. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Eluent (sometimes spelled eluant) – the solvent or solvent fixure used in elution chromatography and is synonymous with mobile phase. [11] Eluate – the mixture of solute (see Eluite) and solvent (see Eluent) exiting the column. [11] Effluent – the stream flowing out of a chromatographic column.

  7. Supercritical fluid chromatography - Wikipedia

    en.wikipedia.org/wiki/Supercritical_fluid...

    The solvent's elution strength can be increased just by increasing density or alternatively, using a polar co-solvent. In practice, when the fraction of the co-solvent is high, the mobile phase might not be truly at supercritical fluid state, but this terminology is used regardless, and the chromatograms show better elution and higher ...

  8. Hydrophilic interaction chromatography - Wikipedia

    en.wikipedia.org/wiki/Hydrophilic_interaction...

    Other salts, such as 100–300 mM sodium perchlorate, that are soluble in high-organic solvent mixtures (c. 70–90% acetonitrile), can be used to increase the mobile phase polarity to affect elution These salts are not volatile, so this technique is less useful with a mass spectrometer as the detector. Usually a gradient (to increasing amounts ...

  9. Countercurrent distribution - Wikipedia

    en.wikipedia.org/wiki/Countercurrent_distribution

    Countercurrent distribution is a separation process that is founded on the principles of liquid–liquid extraction where a chemical compound is distributed (partitioned) between two immiscible liquid phases (oil and water for example) according to its relative solubility in the two phases.