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A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water).
A Gram stain of mixed Staphylococcus aureus (S. aureus ATCC 25923, gram-positive cocci, in purple) and Escherichia coli (E. coli ATCC 11775, gram-negative bacilli, in red), the most common Gram stain reference bacteria. Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups ...
Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye.
The mechanism of action of the Ziehl-Neelsen stain is not completely understood, but it is thought to involve a chemical reaction between the acidic dyes and the cell walls of the bacteria. The acidity of the dyes causes them to bind more strongly to the cell walls of the bacteria than to other cells or tissues .
If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). [4] Additionally, it can be used for the staining of bacterial spores. Carbol-fuchsin is also used as a topical antiseptic and antifungal. [citation needed]
Staining dyes are dyes used for staining in microbiology and histology. Subcategories. This category has the following 2 subcategories, out of 2 total. I.
Basic fuchsine is a mixture of rosaniline, pararosaniline, new fuchsine and Magenta II. [15] Formulations usable for making of Schiff reagent must have high content of pararosanilin. The actual composition of basic fuchsine tends to somewhat vary by vendor and batch, making the batches differently suitable for different purposes.