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The blood is mixed with a small amount of sterile water to cause hemolysis of the RBCs, yielding free hemoglobin. The sample is next centrifuged for several minutes. The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. The color of the fluid is assessed after 2 minutes.
Potassium hydroxide: a strong base; precursor to most soft and liquid soaps as well as numerous potassium-containing chemicals Potassium permanganate: a strong oxidizing agent; can be used to quantitatively determine the total oxidisable organic material in an aqueous sample; a reagent for the synthesis of organic compounds Pyridinium ...
A clinical chemistry analyzer; hand shows size. Clinical chemistry (also known as chemical pathology, clinical biochemistry or medical biochemistry) is a division in medical laboratory sciences focusing on qualitative tests of important compounds, referred to as analytes or markers, in bodily fluids and tissues using analytical techniques and specialized instruments. [1]
The 3rd analytical group of cations includes ions which form hydroxides that are insoluble even at low concentrations. Cations in the 3rd group are, among others: Fe 2+, Fe 3+, Al 3+, and Cr 3+. The group is determined by making a solution of the salt in water and adding ammonium chloride and ammonium hydroxide. Ammonium chloride is added to ...
An aqueous sample is treated with an equal volume of 1% strong base (sodium or potassium hydroxide) followed by a few drops of aqueous copper(II) sulfate. If the solution turns purple, it contains protein. 5–160 mg/mL can be determined. Peptides with the correct length of at least 3 amino acids are necessary for a significant, measurable ...
Sodium hydroxide, also known as lye and caustic soda, [1] [2] is an inorganic compound with the formula NaOH. It is a white solid ionic compound consisting of sodium cations Na + and hydroxide anions OH −. Sodium hydroxide is a highly corrosive base and alkali that decomposes lipids and proteins at ambient temperatures and may cause severe ...
The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
A blood test is a laboratory analysis performed on a blood sample that is usually extracted from a vein in the arm using a hypodermic needle, or via fingerprick. Multiple tests for specific blood components, such as a glucose test or a cholesterol test , are often grouped together into one test panel called a blood panel or blood work .