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In microbiology, the minimum inhibitory concentration (MIC) is the lowest concentration of a chemical, usually a drug, which prevents visible in vitro growth of bacteria or fungi. [1] [2] MIC testing is performed in both diagnostic [1] [2] and drug discovery laboratories. [3] [4]
Etest is a quantitative technique for determining the antibiotic sensitivity and minimum inhibitory concentration (in μg/mL) of some bacteria including Gram-negative and Gram-positive aerobic bacteria such as Enterobacteriaceae, [2] Pseudomonas, [2] [3] [4] Burkholderia, [2] [5] Staphylococcus, [6] and Enterococcus [7] species and fastidious bacteria, such as anaerobes, N. gonorrhoeae, S ...
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria. [1]: 149 [2]
Bacteria are marked as sensitive, resistant, or having intermediate resistance to an antibiotic based on the minimum inhibitory concentration (MIC), which is the lowest concentration of the antibiotic that stops the growth of bacteria. The MIC is compared to standard threshold values (called "breakpoints") for a given bacterium and antibiotic. [28]
The minimum bactericidal concentration (MBC) is the lowest concentration of an antibacterial agent required to kill a particular bacterium. [1] It can be determined from broth dilution minimum inhibitory concentration (MIC) tests by subculturing to agar plates that do not contain the test agent.
An example of such testing is antibiotic susceptibility testing by measurement of minimum inhibitory concentration which is routinely used in medical microbiology and research. If a suspension used is too heavy or too dilute, an erroneous result (either falsely resistant or falsely susceptible) for any given antimicrobial agent could occur.
Following the allotted time, the plate is removed and checked for bacterial growth. If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred. The results of the broth microdilution method are reported in Minimum Inhibitory Concentration (MIC), or the lowest concentration of antibiotics that stopped ...
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration are used to measure in vitro activity of antimicrobial agents. They are good indicators of antimicrobial potency, but don't give any information relating to time-dependent antimicrobial killing (the so-called post antibiotic effect).