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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
FEMS Yeast Research is a peer-reviewed scientific journal focusing on yeast and yeast-like organisms. The journal was established in 2001. The journal was established in 2001. It is published by Oxford University Press on behalf of the Federation of European Microbiological Societies and the editor-in-chief is John Morrissey.
URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).
Yeast is a monthly peer-reviewed scientific journal published by Wiley-Blackwell. It publishes original research articles, reviews , and short communications on all aspects of Saccharomyces and other clinically important yeasts .
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
Research has since been conducted on "tadpoling", which is a variation of "frogging" that is completed by keeping the test cells diluted in liquid throughout their examination. [ 1 ] A viability assay is an assay that is created to determine the ability of organs , cells or tissues to maintain or recover a state of survival. [ 2 ]
Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking .
Mannans from other yeast, for example candida albicans, have found to cross react with ASCA which suggests that other yeast may induce ASCA associated diseases. ASCA are serological markers of candida albicans infections in humans and animals. [1] Mannan-binding lectin is a lectin produced by humans. In ASCA positive Crohn's disease the serum ...