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In 2013, Hafi et al. [2] developed a novel super-resolution technique through sparse deconvolution of polarization-modulated fluorescent images (SPoD). Because the fluorescent dipole is an inherent feature of fluorescence, and its polarization intensity can be easily modulated with rotating linear polarized excitation, the polarization-based super-resolution technique therefore holds great ...
Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polariser oriented at 90 degrees to the illumination. More complex microscopy techniques which ...
Fluorescence anisotropy or fluorescence polarization is the phenomenon where the light emitted by a fluorophore has unequal intensities along different axes of polarization. Early pioneers in the field include Aleksander Jablonski , Gregorio Weber , [ 1 ] and Andreas Albrecht. [ 2 ]
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to ...
A 2006 study on polarized light coherence led to the development of new supports (the surfs) having contrast amplification properties for standard optical microscopy in cross-polarizer mode. [2] Made of optical layers on an opaque or transparent substrate, these supports do not modify the light polarization after reflection even if the ...
STED is a fluorescence microscopy technique which uses a combination of light pulses to induce fluorescence in a small sub-population of fluorescent molecules in a sample. Each molecule produces a diffraction-limited spot of light in the image, and the centre of each of these spots corresponds to the location of the molecule.
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