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Diaphragm (optics) A Zeiss rotating diaphragm, 1906. [1] One diaphragm with five apertures. In optics, a diaphragm is a thin opaque structure with an opening ( aperture) at its center. The role of the diaphragm is to stop the passage of light, except for the light passing through the aperture. Thus it is also called a stop (an aperture stop, if ...
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
Condenser (optics) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes. The concept is applicable to all kinds ...
The optical microscope, also referred to as a light microscope, is a type of microscopethat commonly uses visible lightand a system of lensesto generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Bright-field microscopy ( BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
Aperture. In biology, the pupil (appearing as a black hole) of the eye is its aperture and the iris is its diaphragm. In humans, the pupil can constrict to as small as 2 mm ( f/ 8.3) and dilate to larger than 8 mm ( f/ 2.1) in some individuals. In optics, the aperture of an optical system (including a system consisted of a single lens) is a ...
Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]
Conoscopic interference pattern. A conoscopic interference pattern or interference figure is a pattern of birefringent colours crossed by dark bands (or isogyres ), which can be produced using a geological petrographic microscope for the purposes of mineral identification and investigation of mineral optical and chemical properties.
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