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  2. Diaphragm (optics) - Wikipedia

    en.wikipedia.org/wiki/Diaphragm_(optics)

    Diaphragm (optics) A Zeiss rotating diaphragm, 1906. [1] One diaphragm with five apertures. In optics, a diaphragm is a thin opaque structure with an opening ( aperture) at its center. The role of the diaphragm is to stop the passage of light, except for the light passing through the aperture. Thus it is also called a stop (an aperture stop, if ...

  3. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  4. Condenser (optics) - Wikipedia

    en.wikipedia.org/wiki/Condenser_(optics)

    Condenser (optics) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes. The concept is applicable to all kinds ...

  5. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscopethat commonly uses visible lightand a system of lensesto generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  6. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    Bright-field microscopy ( BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.

  7. Dark-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Dark-field_microscopy

    Dark-field microscopy (also called dark-ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. Consequently, the field around the specimen (i.e., where there is no specimen to scatter the beam) is generally dark. In optical microscopes a darkfield condenser lens ...

  8. Aperture - Wikipedia

    en.wikipedia.org/wiki/Aperture

    Aperture. In biology, the pupil (appearing as a black hole) of the eye is its aperture and the iris is its diaphragm. In humans, the pupil can constrict to as small as 2 mm ( f/ 8.3) and dilate to larger than 8 mm ( f/ 2.1) in some individuals. In optics, the aperture of an optical system (including a system consisted of a single lens) is a ...

  9. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]

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