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Cassette mutagenesis via Golden Gate Assembly. Cassette mutagenesis is a type of site-directed mutagenesis that uses a short, double-stranded oligonucleotide sequence (gene cassette) to replace a fragment of target DNA. It uses complementary restriction enzyme digest ends on the target DNA and gene cassette to achieve specificity.
Golden Gate assembly involves digesting DNA sequences containing a type IIS restriction enzyme cut site and ligating them together. Golden Gate Cloning or Golden Gate assembly [1] is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase. [2]
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The assembly of billionaires at Trump's inauguration was a display of wealth and power unlike any before. ... lest it contravene Trump's fact-free effusions. ... from the Golden Gate to the U.S ...
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Daniel Voytas is an American geneticist who is Professor of Genetics, Cell Biology and Development at the University of Minnesota [1] and Director of the Center for Precision Plant Genomics. [2]
In bioinformatics, sequence assembly refers to aligning and merging fragments from a longer DNA sequence in order to reconstruct the original sequence. [1] This is needed as DNA sequencing technology might not be able to 'read' whole genomes in one go, but rather reads small pieces of between 20 and 30,000 bases, depending on the technology ...