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This multimeric assembly complex, including p1 encoded by gene 1 (referred to as ZOT, zonula occludens toxin by researchers on Vibrio cholerae phage CTXΦ) is an ATPase containing functional and essential Walker motifs [22] that are thought to mediate the hydrolysis of ATP providing the energy for the assembly of the phage filament. Filamentous ...
Shadowed electron micrograph of unaligned phage. Ff phages (for F specific filamentous phages) is a group of almost identical filamentous phage (genus Inovirus) including phages f1, fd, M13 and ZJ/2, which infect bacteria bearing the F fertility factor.
M13 is one of the Ff phages (fd and f1 are others), a member of the family filamentous bacteriophage ().Ff phages are composed of circular single-stranded DNA (), which in the case of the m13 phage is 6407 nucleotides long and is encapsulated in approximately 2700 copies of the major coat protein p8, and capped with about 5 copies each of four different minor coat proteins (p3 and p6 at one ...
Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions.In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively.
Filamentous phages retard bacterial growth but, contrasting with the lambda phage and the T7 phage, are not generally lytic. Helper phages are usually engineered to package less efficiently (via a defective phage origin of replication) [ 5 ] than the phagemid so that the resultant phage particles contain predominantly phagemid DNA.
Structural model at atomic resolution of bacteriophage T4 [1] The structure of a typical myovirus bacteriophage Anatomy and infection cycle of bacteriophage T4.. A bacteriophage (/ b æ k ˈ t ɪər i oʊ f eɪ dʒ /), also known informally as a phage (/ ˈ f eɪ dʒ /), is a virus that infects and replicates within bacteria and archaea.
M13 filamentous phage containing sequence coding for the first 145 amino acid was later constructed by Messing et al., and α-complementation via the use of a vector was demonstrated by the formation of blue plaques when cells containing the inactive protein were infected by the phage and then grown in plates containing X-gal. [3]
Structure of phage ΦX174 capsid Schematic drawing of a Sinsheimervirus (aka Phix174microvirus) virion. The phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA virus that infects Escherichia coli.