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Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are invisible with a bright-field microscope, as exemplified in the figure. These structures were made visible to earlier microscopists by staining, but this required additional preparation and death of the cells. The phase-contrast ...
In the field of transmission electron microscopy, phase-contrast imaging may be employed to image columns of individual atoms; a more common name is high-resolution transmission electron microscopy. It is the highest resolution imaging technique ever developed, and can allow for resolutions of less than one angstrom (less than 0.1 nanometres).
As living cells are translucent, they must be stained to be visible in a traditional light microscope. Unfortunately, the process of staining cells generally kills them. With the invention of the phase-contrast microscopy it became possible to observe unstained living cells in detail. After its introduction in the 1940s, live-cell imaging ...
L-form bacteria, also known as L-phase bacteria, L-phase variants or cell wall-deficient bacteria (CWDB), are growth forms derived from different bacteria. They lack cell walls . [ 1 ] Two types of L-forms are distinguished: unstable L-forms , spheroplasts that are capable of dividing, but can revert to the original morphology, and stable L ...
Cell biology (also cellular biology or cytology) is a branch of biology that studies the structure, function, and behavior of cells. [1] [2] All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living and functioning of organisms. [3] Cell biology is the study of the structural and functional ...
Digital holographic microscopy thus makes it possible to visualize and quantify transparent objects and is therefore also referred to as quantitative phase-contrast microscopy. Traditional phase contrast or bright field images of living unstained biological cells, Figure 3 (right), have proved themselves to be very difficult to analyze with ...
Quantitative phase contrast microscopy or quantitative phase imaging are the collective names for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object. [1] [2] Translucent objects, like a living human cell, absorb and scatter small amounts of light.
One of the difficulties with high resolution transmission electron microscopy is that image formation relies on phase contrast. In phase-contrast imaging, contrast is not intuitively interpretable, as the image is influenced by aberrations of the imaging lenses in the microscope. The largest contributions for uncorrected instruments typically ...