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2. Objective (optics) - Wikipedia

   en.wikipedia.org/wiki/Objective_(optics)

   One of the most important properties of microscope objectives is their magnification.The magnification typically ranges from 4× to 100×. It is combined with the magnification of the eyepiece to determine the overall magnification of the microscope; a 4× objective with a 10× eyepiece produces an image that is 40 times the size of the object.

3. Magnification - Wikipedia

   en.wikipedia.org/wiki/Magnification

   With any telescope, microscope or lens, a maximum magnification exists beyond which the image looks bigger but shows no more detail. It occurs when the finest detail the instrument can resolve is magnified to match the finest detail the eye can see. Magnification beyond this maximum is sometimes called "empty magnification".

4. High-power field - Wikipedia

   en.wikipedia.org/wiki/High-power_field

   The area provides a reference unit, for example in reference ranges for urine tests. [3]Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based on the degree of differentiation, the average number of mitoses per high-power field, cellularity, pleomorphism, and an estimate of the extent of necrosis (presumably a reflection of rate of growth).

5. Optical microscope - Wikipedia

   en.wikipedia.org/wiki/Optical_microscope

   The actual power or magnification of a compound optical microscope is the product of the powers of the eyepiece and the objective lens. For example a 10x eyepiece magnification and a 100x objective lens magnification gives a total magnification of 1,000×.

6. Abbe sine condition - Wikipedia

   en.wikipedia.org/wiki/Abbe_sine_condition

   When the imaging system obeys the Abbe sine condition, the ratio of the sines of these angles equal the (lateral absolute) magnification of the system. In optics , the Abbe sine condition is a condition that must be fulfilled by a lens or other optical system in order for it to produce sharp images of off-axis as well as on-axis objects.

7. Field of view - Wikipedia

   en.wikipedia.org/wiki/Field_of_view

   In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes. For an objective with magnification , the FOV is related to the Field Number (FN) by

8. Optical sectioning - Wikipedia

   en.wikipedia.org/wiki/Optical_sectioning

   With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives

9. Point spread function - Wikipedia

   en.wikipedia.org/wiki/Point_spread_function

   By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...