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An acousto-optic modulator (AOM), also called a Bragg cell or an acousto-optic deflector (AOD), uses the acousto-optic effect to diffract and shift the frequency of light using sound waves (usually at radio-frequency). They are used in lasers for Q-switching, telecommunications for signal modulation, and in spectroscopy for frequency control.
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...
Biophotonics can also be described as the "development and application of optical techniques, particularly imaging, to the study of biological molecules, cells and tissue". [2] One of the main benefits of using the optical techniques which make up biophotonics is that they preserve the integrity of the biological cells being examined. [3] [4]
AOTF may refer to: Acousto-optic tunable filter, a piezoelectric optical device; American Occupational Therapy Foundation, a non-profit charitable, scientific and educational organization. Admiral of the Fleet, the highest rank in the British Royal Navy; AotF may refer to: The Age of the Fall (AF), a wrestling stable
Live-cell imaging was pioneered in the first decade of the 21st century. One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg. [2] Since then, several microscopy methods have been developed to study living cells in greater detail with ...
The major difference between these two microscopy techniques is that FRAP involves the study of a cell’s ability to recover after a single photobleaching event whereas FLIP involves the study of how the loss of fluorescence spreads throughout the cell after multiple photobleaching events.
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
A scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm]. As with a conventional transmission electron microscope (CTEM), images are formed by electrons passing through a sufficiently thin specimen. However, unlike CTEM, in STEM the electron beam is focused ...