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Minimal essential medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture. [1] It is based on six salts and glucose described in Earle's salts in 1934: calcium chloride , potassium chloride , magnesium sulfate , sodium chloride , sodium ...
A chemically defined medium (also known as synthetic medium) is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS) as a source of ...
A bottle of DMEM cell culture medium Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium . Recipes for growth media can vary in pH , glucose concentration, growth factors , and the presence of other nutrients.
MEF cells can be cultured in vitro in DMEM medium with 10% FBS. To transmit MEFs, researches should use trypsin to digest the cells (making them detach from the surface) and transmit 1/5 cells digested into a new dish. [3] [1]
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RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [ 2 ]
Within molecular and cell biology, KOSR, knockout serum replacement, is a defined serum-free formulation optimized to grow and maintain undifferentiated embryonic stem cells in culture. [1]
The complete medium, with OADC enrichment and PANTA antibiotic mixture, is one of the most commonly used liquid media for the cultivation of mycobacteria. All types of clinical specimens, pulmonary as well as extra-pulmonary (except blood and urine ), can be processed for primary isolation in the MGIT tube using conventional methods.