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In cellular biology, P-bodies, or processing bodies, are distinct foci formed by phase separation within the cytoplasm of a eukaryotic cell consisting of many enzymes involved in mRNA turnover. [1] P-bodies are highly conserved structures and have been observed in somatic cells originating from vertebrates and invertebrates , plants and yeast .
Schwann cells or neurolemmocytes (named after German physiologist Theodor Schwann) are the principal glia of the peripheral nervous system (PNS). Glial cells function to support neurons and in the PNS, also include satellite cells, olfactory ensheathing cells, enteric glia and glia that reside at sensory nerve endings, such as the Pacinian corpuscle.
Satellite glial cells, formerly called amphicytes, [1] are glial cells that cover the surface of neuron cell bodies in ganglia of the peripheral nervous system. Thus, they are found in sensory , sympathetic , and parasympathetic ganglia .
The cells after transformation are exposed to the selective media, and only cells containing the plasmid may survive. In this way, the antibiotics act as a filter to select only the bacteria containing the plasmid DNA. The vector may also contain other marker genes or reporter genes to facilitate selection of plasmids with cloned inserts.
The podocyte cell bodies no longer maintain their typical position "floating" within the filtrate above the GBM. Instead, they become broadly adherent to it, resulting in the near-complete obliteration of the subpodocyte space, the region beneath the podocyte cell body and major processes.
Nissl bodies show changes under various physiological conditions and in pathological conditions such as axonotmesis, during which they may dissolve and largely disappear (chromatolysis). If the neuron is successful in repairing the damage, the Nissl bodies gradually reappear and return to their characteristic distribution within the cell. [5]
Nervous tissue, also called neural tissue, is the main tissue component of the nervous system.The nervous system regulates and controls body functions and activity. It consists of two parts: the central nervous system (CNS) comprising the brain and spinal cord, and the peripheral nervous system (PNS) comprising the branching peripheral nerves.
The transformation is usually done by electroporation, which uses electricity to allow the plasmids permeate into the cells. If high expression levels are desired, the P1 lytic replicon can be used in constructs. [5] Electroporation allows for lysogeny of PACs so that they can replicate within cells without disturbing other chromosomes. [1]