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Serology is the scientific study of serum and other body fluids.In practice, the term usually refers to the diagnostic identification of antibodies in the serum. [1] Such antibodies are typically formed in response to an infection (against a given microorganism), [2] against other foreign proteins (in response, for example, to a mismatched blood transfusion), or to one's own proteins (in ...
In immunology, epitope mapping is the process of experimentally identifying the binding site, or epitope, of an antibody on its target antigen (usually, on a protein). [ 1 ] [ 2 ] [ 3 ] Identification and characterization of antibody binding sites aid in the discovery and development of new therapeutics , vaccines , and diagnostics .
Analytes in biological liquids such as serum or urine are frequently measured using immunoassays for medical and research purposes. [1] Immunoassays come in many different formats and variations. Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in the assay.
The dominant research method is the randomised controlled trial. Qualitative research is based in the paradigm of phenomenology, grounded theory, ethnography and others, and examines the experience of those receiving or delivering the nursing care, focusing, in particular, on the meaning that it holds for the individual
The direct attachment of the fluorophore to the antibody reduces the number of steps in the sample preparation procedure, saving time and reducing non-specific background signal during analysis. [12] This also limits the possibility of antibody cross-reactivity, and possible mistakes throughout the process.
The CCC is a nursing terminology specifically developed for computerization: e.g. electronic healthcare information systems (EHR), computer-based patient records (CPR), and Clinical Information Systems (CIS), from research which collected live patient care data. The CCC System describes the six steps of the nursing process: Assessment; Diagnosis
Injection process; Blood serum extraction; An antigen/adjuvant conjugate is injected into an animal of choice to initiate an amplified immune response. After a series of injections over a specific length of time, the animal is expected to have created antibodies against the conjugate.
One method of research for evidence-based practice in nursing is 'qualitative research': The word implies an entity and meanings that are not experimentally examined or measured in terms of quantity, amount, frequency, or intensity. With qualitative research, researchers learn about patient experiences through discussions and interviews.