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Tissue embedded within optimal cutting temperature compound (OCT), mounted on a chuck in a cryostat, and ready for section production. The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] The technical name for this procedure is ...
Various paraffin tissue arrays are now commercially available from many biotech companies. Most of the arrays can be easily made by microarraying instrument (Beecher Instruments Inc.). However, paraffin embedded tissues have limitations. Buffered formalin solutions cross link proteins and nucleic acids when they are used for fix tissues.
This process is called embedding. [22] The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22]
In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical biopsies or tumor samples. These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern.
Prior to cutting by microtome, biological materials are usually placed in a more rigid fixative, in a process known as embedding. This is achieved by the inflow of a liquid substance around the sample, such as paraffin (wax) or epoxy, which is placed in a mold and later hardened to produce a "block" which is readily cut.
Alternatives to paraffin wax include, epoxy, acrylic, agar, gelatin, celloidin, and other types of waxes. [12] [17] In electron microscopy epoxy resins are the most commonly employed embedding media, [9] but acrylic resins are also used, particularly where immunohistochemistry is required.
The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). The tissue preparation starts by collecting the appropriate tissue sections to perform RNA FISH. First, cells, circulating tumor cells (CTCs), formalin-fixed paraffin-embedded (FFPE), or frozen tissue sections are ...
Paraffin impregnation was introduced in 1925, and the embedding of organs in plastic was developed in the 1960s. [citation needed] Body preservation methods current to the 21st century are cryopreservation, which involves the cooling of the body to very low temperatures to preserve the body tissues, plastination, and embalming. [12]
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