Search results
Results from the WOW.Com Content Network
Sectioning intervals can be classified mainly into either: Serial sectioning: obtaining a continuous ribbon of sections from a paraffin block and using all for slides. Step sections: collected at specified depths in the block.
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. [2]
These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Sections from this block are cut using a microtome, mounted on a microscope slide and then analyzed by any method of standard histological analysis. Each microarray block can be cut into 100 – 500 sections, which can be subjected to ...
Various paraffin tissue arrays are now commercially available from many biotech companies. Most of the arrays can be easily made by microarraying instrument (Beecher Instruments Inc.). However, paraffin embedded tissues have limitations. Buffered formalin solutions cross link proteins and nucleic acids when they are used for fix tissues.
Sectioning a tissue can use either the micro tome knife or the razor blade as the cutting blade. [4] The micro tome knife is used for handling sectioning. It is necessary to use a micro tome knife when preparing sections less than 1/1000 micrometers. [29] When using such a knife, the operators must be extremely careful.
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
The tissue is then prepared for viewing under a microscope using either chemical fixation or frozen section. If a large sample is provided e.g. from a surgical procedure then a pathologist looks at the tissue sample and selects the part most likely to yield a useful and accurate diagnosis - this part is removed for examination in a process ...
In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction.It terminates any ongoing biochemical reactions and may also increase the treated tissues' mechanical strength or stability.