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The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.
Agar dilution is one of two methods (along with broth dilution) used by researchers to determine the minimum inhibitory concentration (MIC) of antibiotics. It is the dilution method most frequently used to test the effectiveness of new antibiotics when a few antibiotics are tested against a large panel of different bacteria.
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
The spread plate method wherein the sample (in a small volume) is spread across the surface of a nutrient agar plate and allowed to dry before incubation for counting. [11] The membrane filter method wherein the sample is filtered through a membrane filter, then the filter placed on the surface of a nutrient agar plate.
Cell spreaders. In microbiology, a cell spreader or plate spreader is a tool used to smoothly spread cells and bacteria on a culture plate, such as a petri dish.. Cell spreaders can be made from glass, plastic, or metal, and come in various shapes.
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
A typical Petrifilm plate has a 10 cm(H) × 7.5 cm(W) bottom film which contains a foam barrier accommodating the plating surface, the plating surface itself (a circular area of about 20 cm 2), and a top film which encloses the sample within the Petrifilm. A 1 cm × 1 cm yellow grid is printed on the back of the plate to assist enumeration.
The ground is spread over the plate as evenly as possible using a roller. Once applied the etching plate is removed from the hot-plate and allowed to cool which hardens the ground. After the ground has hardened the artist "smokes" the plate, classically with 3 beeswax tapers, applying the flame to the plate to darken the ground and make it ...