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Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages).
Nile red (also known as Nile blue oxazone) is formed by boiling Nile blue with sulfuric acid. This produces a mix of Nile red and Nile blue. Nile red is a lipophilic stain; it will accumulate in lipid globules inside cells, staining them red. Nile red can be used with living cells.
They settled on nile red, a compound for dyeing used in biochemistry, as it sounded good and did not sound much like a chemical. A second channel, NileBlue, was created in 2016 to showcase more casual projects than the main channel, and a shorts channel, NileRed 2 (formerly NileRed Shorts), was created in 2021.
Humans are exposed to toxic chemicals and microplastics at all stages in the plastics life cycle. Microplastics effects on human health are of growing concern and an area of research. The tiny particles known as microplastics (MPs), have been found in various environmental and biological matrices, including air, water, food, and human tissues.
Category: Vital stains. ... Nile blue; Nile red; S. Safranin; T. Trypan blue This page was last edited on 30 March 2013, at 19:40 (UTC). Text is available under ...
Microplastics are fragments of plastic less than 5 mm (0.20 in) in length, according to the U.S. National Oceanic and Atmospheric Administration (NOAA) [1] [2] and the European Chemicals Agency. [3] US EPA researchers define microplastics as plastic particles ranging in size from 1 nanometer up to 5 mm, which is 5 million nanometers. For ...
The lipid content of the macrophages can be demonstrated using a lipid targeting stain like Oil Red O or Nile red. [ 2 ] [ 4 ] [ 5 ] [ 6 ] Increased levels of lipid-laden alveolar macrophages are associated with various respiratory conditions, including chronic smoking, [ 3 ] gastroesophageal reflux , lipoid pneumonia , fat embolism , [ 7 ...
Sudan stains and Sudan dyes are synthetic organic compounds that are used as dyes for various plastics (plastic colorants) and are also used to stain sudanophilic biological samples, usually lipids. Sudan II , Sudan III , Sudan IV , Oil Red O , and Sudan Black B are important members of this class of compounds (see images below).