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Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages).
They settled on nile red, a compound for dyeing used in biochemistry, as it sounded good and did not sound much like a chemical. A second channel, NileBlue, was created in 2016 to showcase more casual projects than the main channel, and a shorts channel, NileRed 2 (formerly NileRed Shorts), was created in 2021.
Nile red (also known as Nile blue oxazone) is formed by boiling Nile blue with sulfuric acid. This produces a mix of Nile red and Nile blue. Nile red is a lipophilic stain; it will accumulate in lipid globules inside cells, staining them red. Nile red can be used with living cells.
Plants are important for the environment and ecosystems so the plastics are damaging to plants and organisms living in these ecosystems. [102] Microplastics alter soil biophysical properties which affect the quality of the soil. This affects soil biological activity, biodiversity and plant health. Microplastics in the soil alter a plant's growth.
Microplastics are tiny pieces of plastic that are no larger than five millimeters (0.2 inches)—about the size of a single sesame seed—and form when larger plastics break down, either by ...
With respect to both plant microtechnique and animal microtechnique, four types of methods are commonly used, which are whole mounts, smears, squashes, and sections, in recent micro experiments. [2] Plant microtechnique contains direct macroscopic examinations, freehand sections, clearing, maceration, embedding, and staining. [ 3 ]
A diaphonized mirror dory.The bones are dyed red and the cartilage is dyed blue. Diaphonized veiled chameleon. Field Museum of Natural History, Chicago.. Diaphonization (or diaphonisation), also known as clearing and staining, is a staining technique used on animal specimens that first renders the body of the animal transparent by bathing it in trypsin, and then stains the bones and cartilage ...
Likewise, humans are also susceptible to microplastic consumption. People who eat seafood also eat some of the microplastics that were ingested by marine life. Oysters and clams are popular vehicles for human microplastic consumption. [180] Animals who are within the general vicinity of the water are also affected by the plastic in the ocean.