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The count represents the number of colony forming units (cfu) per g (or per ml) of the sample. A TVC is achieved by plating serial tenfold dilutions of the sample until between 30 and 300 colonies can be counted on a single plate. The reported count is the number of colonies counted multiplied by the dilution used for the counted plate
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
The total number of colonies is referred to as the total viable count (TVC). The unit of measurement is cfu/ml (or colony forming units per millilitre) and relates to the original sample. Calculation of this is a multiple of the counted number of colonies multiplied by the dilution used. [citation needed]
When the method only recounts living organisms is called "viable count". [2] There are many methods for the quantification of microorganisms, including microscopy methods, Coulter counter, Mass Spectrometry (for estimating cell mass), and Cell Culture methods which form and grow colonies of bacteria.
The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...
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The HP-12C is a financial calculator made by Hewlett-Packard (HP) and its successor HP Inc. as part of the HP Voyager series, introduced in 1981.It is HP's longest and best-selling product and is considered the de facto standard among financial professionals.
Operating profit = gross profit – total operating expenses Net profit = operating profit – taxes – interest Net profit = net sales – cost of goods sold – operating expense – taxes – interest