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Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range. Boric acid has a pK a of 9.14 at 25 °C.
Borate anions are largely in the form of the undissociated acid in aqueous solution at physiological pH. No further metabolism occurs in either animals or plants. In animals, boric acid/borate salts are essentially completely absorbed following oral ingestion. Absorption occurs via inhalation, although quantitative data are unavailable.
LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Lithium borate ( lithium hydroxide monohydrate and boric acid ).
Buffer capacity falls to 33% of the maximum value at pH = pK a ± 1, to 10% at pH = pK a ± 1.5 and to 1% at pH = pK a ± 2. For this reason the most useful range is approximately p K a ± 1. When choosing a buffer for use at a specific pH, it should have a p K a value as close as possible to that pH.
This page contains tables of azeotrope data for various binary and ternary mixtures of solvents. The data include the composition of a mixture by weight (in binary azeotropes, when only one fraction is given, it is the fraction of the second component), the boiling point (b.p.) of a component, the boiling point of a mixture, and the specific gravity of the mixture.
Substance Formula 0 °C 10 °C 20 °C 30 °C 40 °C 50 °C 60 °C 70 °C 80 °C 90 °C 100 °C Barium acetate: Ba(C 2 H 3 O 2) 2: 58.8: 62: 72: 75: 78.5: 77: 75
Disodium octaborate is a borate of sodium, a chemical compound of sodium, boron, and oxygen — a salt with elemental formula Na 2 B 8 O 13 or (Na +) 2 [B 8 O 13] 2−, also written as Na 2 O·4B 2 O 3. It is a colorless crystalline solid, soluble in water.
It is made up of sodium borate, usually 1–10 mM at pH 8.0. It has a lower conductivity, produces sharper bands, and can be run at higher speeds than can gels made from TBE buffer or TAE buffer (5–35 V/cm as compared to 5–10 V/cm). At a given voltage, heat will be generated and thus the gel will be heated.