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Individuals with an inherited impairment in DNA repair capability are often at increased risk of cancer. [45] When a mutation is present in a DNA repair gene, the repair gene will either not be expressed or be expressed in an altered form. Then the repair function will likely be deficient, and, as a consequence, damages will tend to accumulate.
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. [1] In human cells, both normal metabolic activities and environmental factors such as radiation can cause DNA damage, resulting in tens of thousands of individual molecular lesions per cell per day. [2]
Currently, off-target effects of CRISPRi are minimal, and show a reduced response and sensitivity to single-base mismatches. [44] Importantly, when non-specific effects do inevitably occur they are reversible, time-dependent, and less damaging than DNA editing, making them effective alternatives that can limit the off-target burden when possible.
Consumer Reports states that PriceGrabber places the ads and pays a percentage of referral fees to CR, [25] who has no direct relationship with the retailers. [26] Consumer Reports publishes reviews of its business partner and recommends it in at least one case. [27]
Another source of genome instability may be epigenetic or mutational reductions in expression of DNA repair genes. Because endogenous (metabolically-caused) DNA damage is very frequent, occurring on average more than 60,000 times a day in the genomes of human cells, any reduced DNA repair is likely an important source of genome instability.
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