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Recently a web-based database of human and mouse housekeeping genes and reference genes/transcripts, named Housekeeping and Reference Transcript Atlas (HRT Atlas), was developed to offer updated list of housekeeping genes and reliable candidate reference genes/transcripts for RT-qPCR data normalization. [1]
This is a list of housekeeping genes and transcripts stably expressed across 52 human tissues and cell types that may be used for reference purposes after empirical validation: [1] Gene Ensembl Transcript no.
Housekeeping and Reference Transcript Atlas (HRT Atlas) [27] web-based tool for searching cell specific candidate reference genes/transcripts suitable for qPCR experiment normalization. HRT Atlas also describes a complete list of human and mouse housekeeping genes and transcripts
•List of human protein-coding genes page 2 covers genes EPHA1–MTMR3 •List of human protein-coding genes page 3 covers genes MTMR4–SLC17A7 •List of human protein-coding genes page 4 covers genes SLC17A8–ZZZ3 NB: Each list page contains 5000 human protein-coding genes, sorted alphanumerically by the HGNC-approved gene symbol. Follow ...
Thus both the number and type of housekeeping genes interrogated by MLST may differ from species to species. For each of these housekeeping genes, the different sequences are assigned as alleles and the alleles at the loci provide an allelic profile. A series of profiles can then be the identification marker for strain typing.
Gene ID DBD Motif status (Feb 2018) (Link to human TFs annotation) IUPAC consensus (from selected PWM) AC008770.3: ENSG00000267179: C2H2 ZF: Likely sequence specific TF according to literature or domain structure – No motif : AC023509.3: ENSG00000267281: bZIP: Known motif – from protein with 100% identical DBD – in vitro : RTGACGTCAY ...
Essential genes include housekeeping genes (critical for basic cell functions) [112] as well as genes that are expressed at different times in the organisms development or life cycle. [113] Housekeeping genes are used as experimental controls when analysing gene expression, since they are constitutively expressed at a relatively constant level.
In mammalian genomes, CpG islands are typically 300–3,000 base pairs in length, and have been found in or near approximately 40% of promoters of mammalian genes. [16] Over 60% of human genes and almost all house-keeping genes have their promoters embedded in CpG islands. [17]