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When stored as a powder at 4 °C or below, IPTG is stable for 5 years. It is significantly less stable in solution; Sigma recommends storage for no more than a month at room temperature. [3] IPTG is an effective inducer of protein expression in the concentration range of 100 μmol/L to 3.0 mmol/L. Typically, a sterile, filtered 1 mol/L solution ...
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [ 6 ]
Often, the plate containing X-Gal also contains IPTG (isopropyl β-D-1-thiogalactopyranoside). IPTG is a chemical structure analogue of lactose. [7] However, IPTG cannot be hydrolyzed by β-galactosidase. IPTG is used as an inducer that binds to lac repressor releasing the DNA and allowing transcription.
The expression of a protein may be tightly controlled, and the protein is only produced in significant quantity when necessary through the use of an inducer. In some systems, however, the protein may be expressed constitutively. Escherichia coli is commonly used as the host for protein production, but other cell types may also be used.
Index inducer or just inducer predictably induce metabolism via a given pathway and are commonly used in prospective clinical drug-drug interaction studies. [ 4 ] Strong, moderate, and weak inducers are drugs that decreases the AUC of sensitive index substrates of a given metabolic pathway by ≥80%, ≥50% to <80%, and ≥20% to <50% ...
The tac promoter finds various applications. The tac promoter/operator (dubbed PTAC) is one of the most widely used expression systems.Ptac is a strong hybrid promoter composed of the –35 region of the trp promoter and the –10 region of the lacUV5 promoter/operator.
Indirect materials cost: Indirect materials cost is the cost associated with consumables, such as lubricants, grease, and water, that are not used as raw materials. Other indirect manufacturing cost: includes machine depreciation, land rent, property insurance, electricity, freight and transportation, or any expenses that keep the factory ...
A ceramic multi-chip module containing four POWER5 processor dies (center) and four 36 MB L3 cache dies (periphery). A multi-chip module (MCM) is generically an electronic assembly (such as a package with a number of conductor terminals or "pins") where multiple integrated circuits (ICs or "chips"), semiconductor dies and/or other discrete components are integrated, usually onto a unifying ...