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Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells [3]). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be ...
The Journal of Bacteriology is a monthly peer-reviewed scientific journal established in 1916. It is published by the American Society for Microbiology and the editor in chief is George A. O'Toole Jr. ( Dartmouth College ).
Colony hybridization is a method of selecting bacterial colonies with desired genes through a straightforward cloning and transfer process. [1] The genes of interest have been added to a bacterial plasmid previously through recombination , allowing genes from other organisms to be analyzed within a bacterial colony.
The second reason is that haploid cells of one mating type, upon cell division, often produce cells of the opposite mating type. An analysis of the ancestry of natural S. cerevisiae strains concluded that outcrossing occurs very infrequently (only about once every 50,000 cell divisions). [ 32 ]
One method of microbiological culture is liquid culture, in which the desired organisms are suspended in a liquid nutrient medium, such as Luria broth, in an upright flask. This allows a scientist to grow up large amounts of bacteria or other microorganisms for a variety of downstream applications.
For example, one study [12] used serial passage in baboons to create a strain of HIV-2 that is particularly virulent to baboons. Typical strains of HIV-2 only infect baboons slowly. [12] This specificity makes it challenging for scientists to use HIV-2 in animal models of HIV-1, because the animals in the model will only show symptoms slowly.
Cell engineering often makes use of recombinant DNA technology to achieve these modifications as well as closely related tissue engineering methods. [1] Cell engineering can be characterized as an intermediary level in the increasingly specific disciplines of biological engineering which includes organ engineering, tissue engineering , protein ...
[21] [22] As a result, 16S rRNA gene sequencing has become prevalent in medical microbiology as a rapid and cheap alternative to phenotypic methods of bacterial identification. [23] Although it was originally used to identify bacteria, 16S sequencing was subsequently found to be capable of reclassifying bacteria into completely new species ...