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Gel permeation chromatography (GPC) [1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers.
Gel permeation chromatography is often used in combination with multi-angle light scattering (MALS), Low-angle laser light scattering (LALLS) and/or viscometry for an absolute determination (i.e., independent of the chromatographic separation details) of the molecular weight distribution as well as the branching ratio and degree of long chain ...
Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers . [ 3 ]
Thermoresponsive polymers can be used as stationary phase in liquid chromatography. [1] ... of modified applications was a gel permeation chromatography technique ...
Size-exclusion chromatography (SEC) is also known as gel permeation chromatography (GPC) or gel filtration chromatography and separates molecules according to their size (or more accurately according to their hydrodynamic diameter or hydrodynamic volume). Smaller molecules are able to enter the pores of the media and, therefore, molecules are ...
Gel permeation chromatography (GPC)—separation of synthetic polymers (aqueous or organic soluble). GPC is a powerful technique for polymer characterization using primarily organic solvents. Gel filtration chromatography (GFC)—separation of water-soluble biopolymers. GFC uses primarily aqueous solvents (typically for aqueous soluble ...
The company's first major break came when Dow Chemical bought one of Waters’ first gel permeation chromatography instruments, Dow Chemical made an additional investment of $400,000. By 1979, Dow Chemical had attained nearly 25 percent ownership in Waters.
The rejection can be determined in various ways and provides an indirect measurement of the pore size. One possibility is the filtration of macromolecules (often dextran, polyethylene glycol or albumin), another is measurement of the cut-off by gel permeation chromatography. These methods are used mainly to measure membranes for ultrafiltration ...
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