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Salmonella spp appear to be yellow or colourless colonies, often with a dark centre. As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be ...
Salmonella species: red colonies, some with black centers. The agar itself will turn red due to the presence of Salmonella type colonies. Shigella species: red colonies. Coliforms: yellow to orange colonies. Pseudomonas aeruginosa: pink, flat, rough colonies. This type of colony can be easily mistaken for Salmonella due to the color similarities.
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars). Bismuth sulfite agar is a type of agar media used to isolate Salmonella species.
Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria.
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Selenite broth is used as a selective medium for the isolation of Salmonella species. [1] Selenite broth was originated by Leifson, [2] while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Selenite broth is used as a selective enrichment for the cultivation of Salmonella spp. that may be present in small ...
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]