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3-Amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry, specifically for visualizing sections stained with HRP-conjugated secondary antibodies. [1]
Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
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The compound's structure is based on the indole structure, but in which a second benzene ring is fused onto the five-membered ring at the 2–3 position of indole (equivalent to the 9a–4a double bond in carbazole, respectively). Carbazole is a constituent of tobacco smoke. [3]
3-amino-9-ethylcarbazole, an immunohistochemistry stain (chromogen) Abstract elementary class, in mathematical logic; Acoustic echo cancellation, in telephony; Automatic exposure control, in radiography; Anion exchange capacity, in pedology (soil sciences)
It is prepared by condensation of chloranil and 3-amino-N-ethylcarbazole. It has a centrosymmetric angular structure. [ 2 ] For many years, the structure was assigned, incorrectly, as having a "linear structure" ( EC no. 228-767-9, CAS RN 6358-30-1) which differ in terms of the carbazole ring fusion .
Epinastine (brand names Alesion, Elestat, Purivist, Relestat) is a second-generation antihistamine and mast cell stabilizer that is used in eye drops to treat allergic conjunctivitis.
In enzymology, a zeatin 9-aminocarboxyethyltransferase (EC 2.5.1.50) is an enzyme that catalyzes the chemical reaction. O-acetyl-L-serine + zeatin lupinate + acetate. Thus, the two substrates of this enzyme are O-acetyl-L-serine and zeatin, whereas its two products are lupinate and acetate.