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DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "sequences"), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the UPC barcode ...
The Barcode of Life Data System (commonly known as BOLD or BOLDSystems) is a web platform specifically devoted to DNA barcoding. [ 1 ] [ 2 ] It is a cloud-based data storage and analysis platform developed at the Centre for Biodiversity Genomics in Canada.
Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and sequencing of specific DNA sequences and their comparison with sequences deposited in a DNA barcode database such as the ISHAM reference database, [1] or the Barcode of Life Data System (BOLD). In this attempt, DNA barcoding ...
DNA barcoding of cyanobacteria can be applied in various ecological, evolutionary and taxonomical studies. Some examples include assessment of cyanobacterial diversity and community structure, [ 34 ] identification of harmful cyanobacteria in ecologically and economically important waterbodies [ 35 ] and assessment of cyanobacterial symbionts ...
The Consortium for the Barcode of Life (CBOL) was an international initiative dedicated to supporting the development of DNA barcoding as a global standard for species identification. [1] CBOL's Secretariat Office is hosted by the National Museum of Natural History, Smithsonian Institution, in Washington, DC.
The proper identification of fish specimens with DNA barcoding methods relies heavily on the quality and species coverage of available sequence databases. A fish reference database is an electronic database that typically contains DNA barcodes, images, and geospatial coordinates of examined fish specimens.
The key concept for barcoding macroinvertebrates, is proper selection of DNA markers (DNA barcode region) to amplify appropriate gene regions, using PCR techniques. The DNA barcode region needs to be ideally conserved within a species, but variable among different (even closely related) species and therefore, its sequence should serve as a ...
Metabarcoding is the barcoding of DNA/RNA (or eDNA/eRNA) in a manner that allows for the simultaneous identification of many taxa within the same sample. The main difference between barcoding and metabarcoding is that metabarcoding does not focus on one specific organism, but instead aims to determine species composition within a sample.