Search results
Results from the WOW.Com Content Network
ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer. The amount of light produced is directly proportional to the amount of ATP present in the sample. ATP tests can be used to: Control biological treatment reactors; Guide biocide dosing programs
Microtox is an in vitro testing system which uses bioluminescent bacteria (Allivibrio fischeri, formerly known as Vibrio fischeri) to detect toxic substances in different substrates such as water, air, soils and sediments. [1] Allivibrio fischeri are non-pathogenic, marine, bacteria that luminesce as a natural part of their metabolism. [2]
Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology.
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein.The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. [1]
Imaging of engineered E. coli Nissle 1917 in the mouse gut. Bioluminescence imaging (BLI) is a technology developed over the past decades (1990's and onward). [1] [2] [3] [when?] that allows for the noninvasive study of ongoing biological processes [4] [1] [5] [6] [7] Recently, bioluminescence tomography (BLT) has become possible and several systems have become commercially available.
This form of bioluminescence imaging allows scientists to test drugs in the pre-clinical stages cheaply. Another protein, aequorin, found in certain jellyfish, produces blue light in the presence of calcium. It can be used in molecular biology to assess calcium levels in cells.
Bioluminescence is the emission of light during a chemiluminescence reaction by living organisms. [1] Bioluminescence occurs in diverse organisms ranging from marine vertebrates and invertebrates , as well as in some fungi , microorganisms including some bioluminescent bacteria , dinoflagellates and terrestrial arthropods such as fireflies .
Four genes have been identified as members of the K ATP gene family. The sur1 and kir6.2 genes are located in chr11p15.1 while kir6.1 and sur2 genes reside in chr12p12.1. The kir6.1 and kir6.2 genes encode the pore-forming subunits of the K ATP channel, with the SUR subunits being encoded by the sur1 (SUR1) gene or selective splicing of the sur2 gene (SUR2A and SUR2B).