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Depiction of a red blood cell in an isotonic solution. A solution is isotonic when its effective osmole concentration is the same as that of another solution. In biology, the solutions on either side of a cell membrane are isotonic if the concentration of solutes outside the cell is equal to the concentration of solutes inside the cell.
Water can enter the cell by diffusion through the cell membrane or through selective membrane channels called aquaporins, which greatly facilitate the flow of water. [1] It occurs in a hypotonic environment, where water moves into the cell by osmosis and causes its volume to increase to the point where the volume exceeds the membrane's capacity ...
When the cell is in a hypertonic solution, water flows out of the cell, which decreases the cell's volume. When in a hypotonic solution, water flows into the membrane and increases the cell's volume, while in an isotonic solution, water flows in and out of the cell at an equal rate. [4] Turgidity is the point at which the cell's membrane pushes ...
Osmotic pressure is an important factor affecting biological cells. [4] Osmoregulation is the homeostasis mechanism of an organism to reach balance in osmotic pressure. Hypertonicity is the presence of a solution that causes cells to shrink. Hypotonicity is the presence of a solution that causes cells to swell.
Ringer's solution. Ringer's solution is a solution of several salts dissolved in water for the purpose of creating an isotonic solution relative to the body fluids of an animal. Ringer's solution typically contains sodium chloride, potassium chloride, calcium chloride and sodium bicarbonate, with the last used to buffer the pH.
In hypertonic solutions water flows out of the cell and the cell shrinks (plasmolysis). In hypotonic solutions, water flows into the cell and the cell swells (turgescence). Osmotic shock or osmotic stress is physiologic dysfunction caused by a sudden change in the solute concentration around a cell, which causes a rapid change in the movement ...
A plant cell in hypotonic solution will absorb water by endosmosis, so that the increased volume of water in the cell will increase pressure, making the protoplasm push against the cell wall, a condition known as turgor. Turgor makes plant cells push against each other in the same way and is the main line method of support in non-woody plant ...
In the first step, the liver is placed in an isotonic solution, in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent. Next, a solution containing collagenase is added to separate the hepatocytes from the liver stroma. This process creates a suspension of hepatocytes, which can be seeded in ...