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The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis. Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream. [1]
Colorimetry is "the science and technology used to quantify and describe physically the human color perception". [1] It is similar to spectrophotometry, but is distinguished by its interest in reducing spectra to the physical correlates of color perception, most often the CIE 1931 XYZ color space tristimulus values and related quantities.
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
CIELAB D50 is available in ICC profiles as a profile connection space named "Lab color space". [2] CIELAB (any white point) is a supported color space in TIFF image files. [17] CIELAB (any white point) is available in PDF documents, where it is called the "Lab color space". [18] [19] CIELAB is an option in Digital Color Meter on macOS described ...
"ColorChecker RGB Summaries, Spreadsheets and Lab TIFF File". brucelindbloom.com. A page showing RGB values for color patches in various RGB color spaces, based on the applet described above, and a set of Excel spreadsheets for comparing these numbers to those in a digital camera or scanner image of the ColorChecker.
The type of color profile that is typically used is called an ICC profile. A cross-platform view of color management is the use of an ICC-compatible color management system. The International Color Consortium (ICC) is an industry consortium that has defined: an open standard for a Color Matching Module (CMM) at the OS level; color profiles for:
The color matching functions are proportional to the intensities of primaries needed to match the monochromatic test color at the wavelength shown on the horizontal scale. In 1931 the International Commission on Illumination (CIE) published the CIE 1931 color spaces which define the relationship between the visible spectrum and human color vision.