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The STRENDA Guidelines [5] propose those minimum information that is needed to comprehensively report kinetic and equilibrium data from investigations of enzyme activities including corresponding experimental conditions. This minimum information is suggested to be addressed in a scientific publication when enzymology research data is reported ...
Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. There are other ways of mapping features on DNA for longer length DNA molecules, such as mapping by transduction. [1]
The cloned enzyme donor immunoassay (CEDIA) involves genetically engineering an enzyme (e.g., beta-galactosidase) into two inactive fragments: a small enzyme donor (ED) conjugated with the drug analog, and a larger enzyme acceptor (EA). When the two fragments associate, the full enzyme converts a substrate into a cleaved colored product.
Human enzymes start to denature quickly at temperatures above 40 °C. Enzymes from thermophilic archaea found in the hot springs are stable up to 100 °C. [13] However, the idea of an "optimum" rate of an enzyme reaction is misleading, as the rate observed at any temperature is the product of two rates, the reaction rate and the denaturation rate.
Gel zymography is often used for the detection and analysis of enzymes produced by microorganisms. [7] This has led to variations on the standard protocol e.g. mixed-substrate zymography. [2] Reverse zymography copolymerizes both the substrate and the enzyme with the acrylamide, and is useful for the demonstration of enzyme inhibitor activity ...
The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions. GSTs can constitute up to 10% of cytosolic protein in some mammalian organs. [ 5 ] [ 6 ] GSTs catalyse the conjugation of GSH—via a sulfhydryl group—to electrophilic centers on a wide variety of substrates in order to make the compounds ...
The technique utilizes the uidA gene of Escherichia coli, which codes for the enzyme, β-glucuronidase; [3] this enzyme, when incubated with specific colorless or non-fluorescent substrates, can convert them into stable colored or fluorescent products. [4] The presence of the GUS-induced color indicates where the gene has been actively expressed.
Beadle wrote in 1966, that after reading the 1951 Cold Spring Harbor Symposium on Genes and Mutations, he had the impression that supporters of the one gene–one enzyme hypothesis “could be counted on the fingers of one hand with a couple of fingers left over.” [10] By the early 1950s, most biochemists and geneticists considered DNA the ...