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In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.
The negatively charged cell wall of many microorganisms attracts the positively charged chromophore which causes the specimen to absorb the stain giving it the color of the stain being used. Positive staining is more commonly used than negative staining in microbiology. The different types of positive staining are listed below. [1]
ZOE can be used as a dental filling material or dental cement in dentistry. [ 1 ] [ 2 ] It is often used in dentistry when the decay is very deep or very close to the nerve or pulp chamber. Because the tissue inside the tooth, i.e. the pulp , reacts badly to the drilling stimulus (heat and vibration), it frequently becomes severely inflamed and ...
[1] [2] The microbe was first described by James Kilian Clarke in 1924. [ 3 ] This bacterium, along with the closely related species Streptococcus sobrinus , can cohabit the mouth: Both contribute to oral disease, and the expense of differentiating them in laboratory testing is often not clinically necessary.
1% and 2% uranyl acetate solutions are used as an indicator, and a titrant in stronger concentrations in analytical chemistry, as it forms an insoluble salt with sodium (the vast majority of sodium salts are water-soluble). Uranyl acetate solutions show evidence of being sensitive to light, especially UV, and will precipitate if exposed.
[1] [2] Tooth polishing has little therapeutic value and is usually done as a cosmetic procedure after debridement and before fluoride application. [3] Common practice is to use a prophy cup —a small motorized rubber cup—along with an abrasive polishing compound.
Calculus was a term used for various kinds of stones. This spun off many modern words, including calculate (' use stones for mathematical purposes '), and calculus, which came to be used, in the 18th century, for accidental or incidental mineral buildups in human and animal bodies, like kidney stones and minerals on teeth. [3]
Cross-sectional diagram of a molar tooth. 1: crown, 2: root, 3: enamel, 4: dentin and dentin tubules, 5: pulp chamber, 6: blood vessels and nerve within root canal, 7: periodontal ligament, 8: apex and periapical region, 9: alveolar bone VITA classical A1-D4 shade guide arranged according to value VITA classical A1-D4 shade guide arranged according to chroma; A: red-brown, B: red-yellow, C ...