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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
used for bacterial or fungal cultures Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter
Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. Bacteria is introduced via an inoculation needle or a pipette tip being stabbed into the center of the agar. Bacteria grow in the punctured area. [11] Stab cultures are most commonly used for short-term storage or shipment of cultures.
Flaming is done to inoculation loops and straight-wires in microbiology labs for streaking. Leaving the loop in the flame of a Bunsen burner or alcohol burner until it glows red ensures that any infectious agent is inactivated or killed. This is commonly used for small metal or glass objects, but not for large objects (see Incineration below ...
Some genera have been divided based upon the presence or absence of motility. Motility is determined by using a motility medium. The ingredients include motility test medium, nutrient broth powder, NaCl and distilled water. An inoculating needle (not a loop) is used to insert the bacterial sample.
Wet each disk with about four inoculating loops of deionized water. Use a loop to aseptically transfer a large mass of pure bacteria to the disk. Observe the disk for up to three minutes. If the area of inoculation turns dark-blue to maroon to almost black, then the result is positive.
An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [ 1 ] [ full citation needed ] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [ 1 ]