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Mordants are chemical agents which have power of making dyes to stain materials which otherwise are unstainable Mordants are classified into two categories: a) Basic mordant: React with acidic dyes e.g. alum, ferrous sulfate, cetylpyridinium chloride etc. b) Acidic mordant : React with basic dyes e.g. picric acid, tannic acid etc.
Counterstain, which is usually positively charged safranin or basic fuchsine, is applied last to give decolorized gram-negative bacteria a pink or red color. [3] [20] Both gram-positive bacteria and gram-negative bacteria pick up the counterstain. The counterstain, however, is unseen on gram-positive bacteria because of the darker crystal ...
Basic dyes are also used in the coloration of paper. Direct or substantive dyeing is normally carried out in a neutral or slightly alkaline dye bath, at or near boiling point, with the addition of either sodium chloride (NaCl) or sodium sulfate (Na 2 SO 4) or sodium carbonate (Na 2 CO 3). Direct dyes are used on cotton, paper, leather, wool ...
It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin. The name basophilic refers to the characteristic of these structures to be stained very well by basic dyes. This can be explained by their ...
The mechanism of action of the Ziehl-Neelsen stain is not completely understood, but it is thought to involve a chemical reaction between the acidic dyes and the cell walls of the bacteria. The acidity of the dyes causes them to bind more strongly to the cell walls of the bacteria than to other cells or tissues. This results in the selective ...
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. [ 1 ] It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods.
Endospore stain on Bacillus subtilis.The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1]
Toluidine blue stain in a vasculitic peripheral neuropathy. Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. [2] It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images.