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  2. Flow-FISH - Wikipedia

    en.wikipedia.org/wiki/Flow-FISH

    Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...

  3. Viability assay - Wikipedia

    en.wikipedia.org/wiki/Viability_assay

    Flow cytometry using 7-Aminoactinomycin D (7-AAD), wherein a lower signal indicates viable cells. Therefore, this case shows good viability (viability of the cells in flow cytometry should be around 95% but not less than 90%. [8]). Cytolysis or membrane leakage: This category includes the lactate dehydrogenase assay. Assays such as these ...

  4. Cell cycle analysis - Wikipedia

    en.wikipedia.org/wiki/Cell_cycle_analysis

    Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).

  5. TUNEL assay - Wikipedia

    en.wikipedia.org/wiki/TUNEL_assay

    The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al. [4] Concurrently, the avidin-peroxidase labeling assay applicable for light absorption microscope was described by Gavrieli et al. [5] Since 1992 the TUNEL has become one of the main methods for ...

  6. 7-Aminoactinomycin D - Wikipedia

    en.wikipedia.org/wiki/7-aminoactinomycin_D

    7-AAD is also used as a cell viability stain. Cells with compromised membranes will stain with 7-AAD, while live cells with intact cell membranes will remain dark. Viability of the cells in flow cytometry should be around 95% but not less than 90%. [4] Flow cytometry using 7-AAD, wherein a lower signal indicates viable cells. Therefore, this ...

  7. ‘Yellowstone’ fans all have the same question after the ...

    www.aol.com/yellowstone-fans-same-finale...

    The "Yellowstone" Season 5 finale just left viewers wanting more and they may just get their wish.On Dec. 15, the popular series wrapped up its fifth season with an explosive finale that killed ...

  8. Fluorescence in situ hybridization - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_in_situ...

    Fibre FISH protocol Archived 2006-10-23 at the Wayback Machine from the Human Genome Project at the Sanger Centre; CARD-FISH, BioMineWiki Archived 2020-07-28 at the Wayback Machine; Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different Fluorochromes; FISH technical notes and protocols from GeneDetect.com

  9. Tragic discovery in Oregon: Missing hiker’s body found ...

    www.aol.com/news/tragic-discovery-oregon-missing...

    The search for missing hiker Susan Lane-Fournier, 61, took a tragic turn after her body was found over the weekend in Welches, Oregon, an unincorporated community at the base of Mount Hood.