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The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
A TSI slant is often used to distinguish nonfermenting Pseudomonas species from enteric pathogens in faecal specimens. [ citation needed ] When P. aeruginosa is isolated from a normally sterile site (blood, bone, deep collections), it is generally considered dangerous, and almost always requires treatment.
Pseudomonas infection refers to a disease caused by one of the species of the genus Pseudomonas. P. aeruginosa is a germ found in the environment and it is an opportunistic human pathogen most commonly infecting immunocompromised patients, such as those with cancer , diabetes , cystic fibrosis , [ 1 ] severe burns, AIDS , [ 2 ] or people who ...
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton, who were microbiologists working at Harvard University.
Cetrimide also enhances the production of Pseudomonas pigments such as pyocyanin and pyoverdine, which show a characteristic blue-green and yellow-green colour, respectively. [ 3 ] [ 4 ] Cetrimide agar is widely used in the examination of cosmetics, pharmaceuticals and clinical specimens to test for the presence of Pseudomonas aeruginosa .
Thayer–Martin agar (or Thayer–Martin medium, or VPN agar) is a Mueller–Hinton agar with 5% chocolate sheep blood and antibiotics. It is used for culturing and primarily isolating pathogenic Neisseria bacteria , including Neisseria gonorrhoeae and Neisseria meningitidis , as the medium inhibits the growth of most other microorganisms.
Klebsiella oxytoca on agar plate. Klebsiella oxytoca is a Gram-negative, rod-shaped bacterium that is closely related to K. pneumoniae, from which it is distinguished by being indole-positive; it also has slightly different growth characteristics in that it is able to grow on melezitose, but not 3-hydroxybutyrate.
For preparation of BCYE + antibiotics, add membrane-filtered antibiotics and mix. For BCYE + albumin agar, dissolve the albumin in distilled water and filter sterilize before addition to the medium. Dispense 20 mL per 15 X 100-mm Petri dish. The medium must be mixed frequently during the pouring to keep the charcoal particles suspended.
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