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Midday leaf gas exchange was measured using a photosynthesis system, which measured net photosynthetic rate, gs, and intercellular CO 2 concentration (). In the same leaves used for gas exchange measurements, chlorophyll a fluorescence parameters (initial, F 0 {\displaystyle \,F_{0}} ; maximum, F m {\displaystyle \,F_{m}} ; and variable, F v ...
Photosynthetic capacity (A max) is a measure of the maximum rate at which leaves are able to fix carbon during photosynthesis. It is typically measured as the amount of carbon dioxide that is fixed per metre squared per second, for example as μmol m −2 sec −1.
A photosynthesis system analysing the photosynthetic rate of a maize leaf. Photosynthesis systems are electronic scientific instruments designed for non-destructive measurement of photosynthetic rates in the field. Photosynthesis systems are commonly used in agronomic and environmental research, as well as studies of the global carbon cycle.
Photosynthesis systems use infrared gas analyzers (IRGAS) for measuring photosynthesis. CO 2 concentration changes in leaf chambers are measured to provide carbon assimilation values for leaves or whole plants. Research has shown that the rate of photosynthesis is directly related to the amount of carbon assimilated by the plant.
The following is a breakdown of the energetics of the photosynthesis process from Photosynthesis by Hall and Rao: [6]. Starting with the solar spectrum falling on a leaf, 47% lost due to photons outside the 400–700 nm active range (chlorophyll uses photons between 400 and 700 nm, extracting the energy of one 700 nm photon from each one)
The pigment in plant leaves, chlorophyll, strongly absorbs visible light (from 400 to 700 nm) for use in photosynthesis. The cell structure of the leaves, on the other hand, strongly reflects near-infrared light (from 700 to 1100 nm). The more leaves a plant has, the more these wavelengths of light are affected.
Photosynthesis measurement systems are not designed to directly measure the amount of light the leaf absorbs, but analysis of chlorophyll fluorescence, P700- and P515-absorbance, and gas exchange measurements reveal detailed information about, e.g., the photosystems, quantum efficiency and the CO 2 assimilation rates.
This is because not all of the water that is taken by the plant is used for transpiration (water taken might be used for photosynthesis or by the cells to maintain turgidity). To measure transpiration rate directly, rather than the rate of water uptake, utilize a scientific instrument which quantifies water transfer at the leaves. However, in ...