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In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
Pages for logged out editors learn more. Contributions; Talk; DNA–DNA hybridisation
Chromosome combing (also known as molecular combing or DNA combing) [1] is a technique used to produce an array of uniformly stretched DNA that is then highly suitable for nucleic acid hybridization studies such as fluorescent in situ hybridisation (FISH) which benefit from the uniformity of stretching, the easy access to the hybridisation target sequences, [2] and the resolution offered by ...
Nucleic acid hybridization, the process of joining two complementary strands of nucleic acids - RNA, DNA or oligonucleotides; In evolutionary algorithms, the merging two or more optimization techniques into a single algorithm Memetic algorithm, a common template for hybridization; In linguistics, the process of one variety blending with another ...
The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation (as well as pore-based methods). However hybridization of oligonucleotides is still used in some sequencing schemes, including hybridization-assisted pore-based sequencing, and ...
It is based on DNA–DNA hybridization studies conducted in the late 1970s and throughout the 1980s. [1] DNA–DNA hybridization is among a class of comparative techniques in molecular biology that produce distance data (versus character data) and that can be analyzed to produce phylogenetic reconstructions only using phenetic tree-building ...
The limitations with the hybridization-ligation assay also apply to the dual ligation assay, with the 5'-end in addition to the 3'-end requiring to have a free hydroxyl (or a phosphate group). Further, T4 DNA ligases are incompatible with ligation of RNA molecules as a donor (i.e. RNA at the 5' end of the ligation).