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The lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in E. coli and many other enteric bacteria.Although glucose is the preferred carbon source for most enteric bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available through the activity of β-galactosidase. [1]
It is encoded by the lacY gene in the lac operon. The LacY gene is a component of the lac operon that encodes lactose permease, a protein responsible for breaking down lactose into glucose and galactose, alongside transacetylase and beta galactosidase.
The lac operon is used in the biotechnology industry for production of recombinant proteins for therapeutic use. The gene or genes for producing an exogenous protein are placed on a plasmid under the control of the lac promoter. Initially the cells are grown in a medium that does not contain lactose or other sugars, so the new genes are not ...
Galactoside permease is a protein coded by the lacY gene of the lac operon, and is found bound to the membrane of a cell for the purpose of binding galactoside molecules that have been solubilized. The protein is part of a system whose main function is to catalyze the accumulation and transport of lactose and other beta-galactosides across the ...
In E. coli, the lacZ gene is the structural gene for β-galactosidase; which is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β-Galactosidase synthesis stops when glucose levels are sufficient. [2] β-Galactosidase has many homologues based on similar sequences.
The lac operon: 1: RNA Polymerase, 2: lac repressor, 3: Promoter, 4: Operator, 5: Lactose, 6: lacZ, 7: lacY, 8: lacA. Top: The gene is essentially turned off.There is no lactose to inhibit the repressor, so the repressor binds to the operator, which obstructs the RNA polymerase from binding to the promoter and making lactase.
The lac operon in the prokaryote E. coli consists of genes that produce enzymes to break down lactose. Its operon is an example of a prokaryotic silencer. The three functional genes in this operon are lacZ, lacY, and lacA. [6] The repressor gene, lacI, will produce the repressor protein LacI which is under allosteric regulation.
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