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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The URA3 marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of C. albicans. [119]
FEMS Yeast Research is a peer-reviewed scientific journal focusing on yeast and yeast-like organisms. The journal was established in 2001. The journal was established in 2001. It is published by Oxford University Press on behalf of the Federation of European Microbiological Societies and the editor-in-chief is John Morrissey.
Another method of selection is the use of certain auxotrophic markers that can compensate for an inability to metabolise certain amino acids, nucleotides, or sugars. This method requires the use of suitably mutated strains that are deficient in the synthesis or utility of a particular biomolecule, and the transformed cells are cultured in a ...
A new trend gaining popularity among people trying to lose weight is microdosing the diabetes medication Ozempic. With approximately 70% of American adults meeting the criteria for being obese or ...
"The Talk" is done talking. CBS' peppy daytime show ended its 15-season run Friday after an hourlong series finale.. The audience gave the show's hosts – Sheryl Underwood, Jerry O'Connell ...
A travel expert revealed the science of why food and drinks taste different on a plane — and what he chooses to order once the beverage cart comes around. Yes, food and drinks taste different on ...