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High sensitivity and specificity, using base qualities at all steps in the alignment. Includes adapter trimming, base quality calibration, Bi-Seq alignment, and options for reporting multiple alignments per read. Use of ambiguous IUPAC codes in reference for common SNPs can improve SNP recall and remove allelic bias. Yes Yes Yes
BBDuk multithreaded tool to trim adapters and filter or mask contaminants based on kmer-matching, allowing a hamming- or edit-distance, as well as degenerate bases. Also performs optimal quality-trimming and filtering, format conversion, contaminant concentration reporting, gc-filtering, length-filtering, entropy-filtering, chastity-filtering ...
Note:If MAST is combining reverse complement DNA strands, the position p-value is not corrected for multiple tests. Sequence p-value: The p-value of a match of a sequence to a motif is defined as the probability of a randomly generated sequence of the same length having a match score at least as large as the largest match score of any position ...
For example, the built-in reverse complement utility reverses the order of characters and replaces each with its complement. [17] The screenshots demonstrate the use of MEGA's reverse complement tool. The original sequence was reversed and each nucleotide was replaced with its complement to produce the reverse complement.
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A single tree can capture up to 250 tons in its lifetime, the equivalent of removing nearly 200 cars from the road for an entire year. But between logging and fires, 95% of California's redwoods ...
Each purine base is the Watson-Crick complement of a unique pyrimidine base (and vice versa) – adenine and thymine form a complementary pair, as do guanine and cytosine. This pairing can be described as follows – ¯ =, ¯ =, ¯ =, ¯ =.
The early returns for Netflix’s NFL games are similarly encouraging, as it reported nearly one third of its global subscribers, coming in from over 200 countries, tuned in for at least part of ...